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This dataset consists of measurements of the digestive gland in the freshwater snail Lymnaea stagnalis orally exposed to uranium. The objective of the study was to assess the morphological effects of uranium on the snail digestive gland by light microscopy. Laboratory-cultured L stagnalis were exposed to either synthetic or natural solid phases of uranium and a subset (6 controls and 6 exposed) were euthanized and fixed in 10% neutral buffered formalin for histopathological assessment. Slides were processed and stained with hematoxalin and eosin as per standard procedure (Luna 1968). The size of each sample, the length and width of digestive gland tubules (5 random glands per sample) and height of digestive cells...
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Reproductive Data for All Nests Monitored; Data for Mayfield Analysis; Eggshell thickness, nestling morphological measurements, nestling plasma stable isotopes, and nestling red blood cell oxidative DNA damage; Egg organochlorine pesticide, total PCB, flame retardants, and PCB toxic equivalent concentrations; Fish capture by osprey; Fish pools and size for contaminant analysis; Fish organochlorine pesticide, total PCB and flame retardants concentrations; Data for estimation of p,p’-DDE and total PCB biomagnification from fish to osprey eggs
These data are comprised of measurements of gross alpha activity, gross beta activity, and radionuclide activities (isotopic U, isotopic Th, Ra-226) in small rodent bodies. Samples were collected at non-mining and U breccia pipe mining sites in various production stages, in the Grand Canyon watershed.
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A seminal question in ecotoxicology is the extent to which contaminant exposure evokes prolonged effects on physiological function and fitness. A series of studies were undertaken with American kestrels ingesting environmentally realistic concentrations of the second-generation anticoagulant rodenticide (SGAR) brodifacoum (BROD). Kestrels fed BROD at 0.3, 1.0 or 3.0 µg/g diet wet wt for 7 d exhibited dose-dependent hemorrhage, histopathological lesions and coagulopathy (prolonged prothrombin and Russell’s viper venom times). Following termination of a 7 d exposure to 0.5 µg BROD/g diet, prolonged blood clotting time returned to baseline values within a week, but BROD residues in liver and kidney (terminal half-life...
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A heterologous thrombin clotting time assay (TCT) was used to measure the time for conversion of fibrinogen to fibrin using commercially available reagents. Human reference material included in the kit was diluted with imidazole buffered saline (IBS; 0.0125M imidazole-0.109 M sodium chloride, pH7.4) to generate a standard curve. Each kestrel plasma sample was thawed at 37ºC and diluted with IBS, and following incubation at 37ºC, the reaction was then initiated by the addition of bovine thrombin reagent supplied with the assay kit, with clotting time measured to 0.1 s. Fibrinogen concentration was determined in a single assay for each of the 3 study trials, with reference samples interspersed among study samples....
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Range finding trial in which kestrels were fed diets containing varying quantities of brodifacoum and signs of intoxication were monitored.
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This dataset consists of concentration and isotopic data for a method designed to isolate and analyze methylmercury. Several matrices (lab-based, and natural) were tested to ensure that the method was capable of recovering methylmercury amendments. Additionally, natural matrices (plankton and sediment) with ambient methylmercury were also analyzed to ensure that the method could be successfully applied to natural matrices. Separation tests for sediment, water, and biotic matrices showed acceptable recoveries (98 ± 5%, n = 54) and reproducible δ202Hg isotope results (2SD < 0.15 ‰) down to 5 ng of MeHg. This dataset is part of a complimentary paper published in Analytical and Bioanalytical Chemistry (https://doi.org/10.1007/s00216-019-02277-0)...
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These data are comprised of measurements of aluminum, iron, cobalt, nickel, copper, zinc, arsenic, selenium, molybdenum, silver, cadmium, thallium, lead, bismuth, thorium, uranium, and mercury in invertebrates, vegetation, and small rodents. Gross alpha activity, gross beta activity, and radionuclide activities (isotopic U, isotopic Th) are also presented for vegetation; radioactivities for small rodents were previously presented at https://doi.org/10.5066/P9HARTQ4. Histopathology results are given for liver and kidney lesions generally associated with metals toxicoses in small rodents. Samples were collected at the Pinenut Mine (active mining) and Arizona 1 Mine (post-production) in the Grand Canyon watershed....
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Two synoptic sampling campaigns were conducted near Breckenridge, Colorado, to quantify metal loading to Illinois Gulch, a tributary of the Blue River. The first campaign, conducted in August 2016, was designed to determine the degree to which Illinois Gulch loses water to the underlying mine workings, and to determine the effect of these losses on observed metal loads. The second campaign, conducted in September 2017, was designed to evaluate metal loading within Iron Springs, a subwatershed that was responsible for the majority of the metal loading observed in 2016. A continuous, instream injection of a sodium bromide (NaBr) tracer was initiated at the head of the respective study reaches several days prior to...
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These data are comprised of measurements of aluminum, iron, cobalt, nickel, copper, zinc, arsenic, selenium, molybdenum, silver, cadmium, thallium, lead, bismuth, thorium, uranium, and mercury in soil, vegetation, and small rodents. Gross alpha activity, gross beta activity, and radionuclide activities (isotopic U, isotopic Th) are also presented for vegetation and soil. Radioactivities for small rodents were previously presented at https://doi.org/10.5066/P99GDFWB. The data also include histopathological analysis of liver tissues collected from small rodents. Samples were collected at the Kanab North Mine and Canyon Mine (post-mining, pre-reclamation) in the Grand Canyon watershed.
This dataset describes histopathological changes in liver, kidney, heart, skeletal muscle and intestine of captive American kestrels exposed to the second-generation anticoagulant rodenticide brodifacoum (BROD). The goal of the study was to determine the toxic range of brodifacoum by feeding birds a diet containing 0.3, 1.0, or 3.0 ug BROD/g wet weight. Birds were necropsied and examined grossly for hemorrhages or anemia, and liver, kidney, heart, pectoral muscle, and intestine was collected for histopathological evaluation. Tissues were scanned at least 100x magnification and all lesions, including hemorrhage, inflammation, and degenerative changes, were described and assigned a morphologic diagnosis with severity,...
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Comma-separated values (.csv) files containing data related to a National-scale assessment of mercury bioaccumulation in the US National Parks using dragonfly larvae as biosentinels through a citizen science framework.
Categories: Data; Tags: Acadia National Park, Allegheny Portage Railroad National Historic Site, Apostle Islands National Lakeshore, Appomattox Court House National Historical Park, Aquatic Biology, All tags...
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Exposure of wildlife to Active Pharmaceutical Ingredients (APIs) is likely to occur but evidence of hazard and risk is limited. One exposure pathway that has received attention is trophic transfer of APIs in a water-fish-osprey food chain. Samples of water, fish plasma and osprey plasma were collected from Delaware River and Bay, and analyzed for 21 APIs. Only 2 of 21 analytes exceeded Method Detection Limits (MDL) in osprey plasma (acetaminophen and diclofenac) with plasma levels typically 2-3 orders of magnitude below human therapeutic concentrations (HTC). We built upon a screening level model used to predict osprey exposure to APIs in Chesapeake Bay and evaluated whether exposure levels could have been predicted...
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Geospatial locations of eDNA sampling sites in the Grand Canyon region near breccia pipe uranium mines
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Recent concern for the adverse effects from neonicotinoid insecticides has centered on risk for insect pollinators in general and bees specifically. However, natural resource managers are also concerned about the risk of neonicotinoids to conservation efforts for the monarch butterfly (Danaus plexippus) and need a tool to estimate risk for wild monarch butterflies exposed to clothianidin. In the present study, monarch butterfly larvae were exposed to contaminated milkweed plants from hatch until pupation, and the effects upon larval survival, larval growth, pupation success, and adult mass were measured. Soils dosed with a granular insecticide product lead to average clothianidin concentrations of 10.8 - 843 ng/g...
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- Observations and treatment of various species of raptorial birds admitted to a rehabilitation facility, and of nestling barn owls observed and sampled in the field - Clotting time parameters (prothrombin time, Russell’s viper venom time, fibrinogen concentration) - Anticoagulant rodenticide residue data
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These data describe the spatio-temporal distribution of dabbling duck species across the continental United States during four biologically relevant seasons. This dataset contains two types of distribution models: (1) probability of presence, and (2) abundance. The model type, species, and season depicted in a raster are defined in the file name. File names begin with either abun (indicating that it is an abundance model) or prob (indicating a probability of occurrence model). Following model type is species, for which there are 10 provided: ABDU (American Black Duck), AMEW (American Wigeon), BWTE (Blue-winged Teal), CITE (Cinnamon Teal), GADW (Gadwall), AGWT (Green-winged Teal), MALL (Mallard), MODU (Mottled...
The risks to wildlife and humans from uranium (U) mining to the Grand Canyon watershed are largely unknown. In addition to U, other co-occurring ore constituents contribute to risks to biological receptors depending on their toxicological profiles. This data was collected to characterize the pre-mining concentrations of total arsenic (As), cadmium (Cd), copper (Cu), lead (Pb), mercury (Hg), nickel (Ni), selenium (Se), thallium (Tl), U, and zinc (Zn); radiation levels; and histopathologies in biota (vegetation, invertebrates, amphibians, birds, and mammals) at the Canyon Mine.


map background search result map search result map Investigation of pharmaceuticals in water, fish, and ospreys nesting in Delaware River and Bay eDNA sampling sites in the Grand Canyon region near breccia pipe uranium mines 2015-2016 Examination of contaminant exposure and reproduction of ospreys (Pandion haliaetus) nesting in Delaware Bay and River in 2015 Chemical analyses and histopathology of small rodents, vegetation, and soil collected from the Kanab North breccia pipe uranium mine in the Grand Canyon watershed Use of blood clotting assays to assess anticoagulant rodenticide exposure and effects in free-ranging birds of prey Synoptic sampling data from Illinois Gulch and Iron Springs near Breckenridge, Colorado, August 2016 and September 2017 Uptake and toxicity of clothianidin to monarch butterflies from milkweed consumption (ver. 2.0, January 2020) Spatio-temporal distribution models for dabbling duck species across the continental United States Growth Characteristics of Syntrophotalea acetylenivorans, strain SFB93, sp. nov., a diazotrophic, acetylenotrophic anaerobe isolated from San Francisco Bay intertidal sediments Brodifacoum toxicity in American kestrels (Falco sparverius) with evidence of increased hazard upon subsequent anticoagulant rodenticide exposure 1. Results of Trial 1 4. Results of Blood Clotting Assays Isolation of Methylmercury Using Distillation and Anion-Exchange Chromatography for Isotopic Analyses in Natural Matrices Data Release Mercury Bioaccumulation in US National Parks Using Dragonfly Larvae as Biosentinels, 2009-2018 Chemical and biological data from acute and chronic nickel and zinc exposure bioassays to two sensitive freshwater benthic invertebrates Chemical analyses and histopathology of organisms and plants collected from breccia pipe uranium mine sites in the Grand Canyon watershed, 2015-2020 Chemical and biological data from acute and chronic nickel and zinc exposure bioassays to two sensitive freshwater benthic invertebrates Synoptic sampling data from Illinois Gulch and Iron Springs near Breckenridge, Colorado, August 2016 and September 2017 Brodifacoum toxicity in American kestrels (Falco sparverius) with evidence of increased hazard upon subsequent anticoagulant rodenticide exposure 1. Results of Trial 1 4. Results of Blood Clotting Assays eDNA sampling sites in the Grand Canyon region near breccia pipe uranium mines 2015-2016 Chemical analyses and histopathology of organisms and plants collected from breccia pipe uranium mine sites in the Grand Canyon watershed, 2015-2020 Chemical analyses and histopathology of small rodents, vegetation, and soil collected from the Kanab North breccia pipe uranium mine in the Grand Canyon watershed Investigation of pharmaceuticals in water, fish, and ospreys nesting in Delaware River and Bay Examination of contaminant exposure and reproduction of ospreys (Pandion haliaetus) nesting in Delaware Bay and River in 2015 Isolation of Methylmercury Using Distillation and Anion-Exchange Chromatography for Isotopic Analyses in Natural Matrices Data Release Use of blood clotting assays to assess anticoagulant rodenticide exposure and effects in free-ranging birds of prey Spatio-temporal distribution models for dabbling duck species across the continental United States Mercury Bioaccumulation in US National Parks Using Dragonfly Larvae as Biosentinels, 2009-2018