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Histopathological assessment of the digestive gland of a freshwater snail (Lymnaea stagnalis) exposed to uranium This dataset consists of measurements of the digestive gland in the freshwater snail Lymnaea stagnalis orally exposed to uranium. The objective of the study was to assess the morphological effects of uranium on the snail digestive gland by light microscopy. Laboratory-cultured L stagnalis were exposed to either synthetic or natural solid phases of uranium and a subset (6 controls and 6 exposed) were euthanized and fixed in 10% neutral buffered formalin for histopathological assessment. Slides were processed and stained with hematoxalin and eosin as per standard procedure (Luna 1968). The size of each sample, the length and width of digestive gland tubules (5 random glands per sample) and height of digestive cells... Categories: Data; Tags: Environmental Health, Lymnaea stagnalis, Madison, Wisconsin, USGS Science Data Catalog (SDC), Wildlife Biology, All tags... Wildlife Disease, digestive gland, uranium, Fewer tags Data describing the use of retention ponds on commercial poultry facilities on Delmarva by wild waterfowl These data support a paired USGS publication and document the use of retention ponds on commercial poultry farms by wild waterfowl. Categories: Data; Tags: Chesapeake Bay, USGS Science Data Catalog (SDC), Wildlife Disease, avian influenza, biota, All tags... domestic poultry, wild waterfowl, Fewer tags In vitro expression, immunogenicity, and efficacy data from recombinant raccoon poxvirus-vectored rabies vaccine candidates tested in mice This work is part of an experimental trial to develop and assess novel recombinant raccoonpox virus (RCN) rabies vaccines in the mouse model, for potential use in bats. Briefly, our research group previously developed a recombinant RCN vaccine candidate expressing a mosaic glycoprotein (MoG) gene that protected mice and big brown bats when challenged with rabies virus (RABV). We developed two new recombinant RCN candidates expressing MoG (RCN-tPA-MoG and RCN-SS-TD-MoG) with the aim of improving RCN-MoG. We assessed and compared in vitro expression, in vivo immunogenicity, and protective efficacy in vaccinated mice challenged intracerebrally with RABV. In this data set, we share results of immunofluorescence assay... Categories: Data; Tags: A/J mice, National Wildlife Health Center, Madison, WI, RABV, USGS Science Data Catalog (SDC), University of Wisconsin-Madison, Madison, WI - Hanson Biomedical Sciences, All tags... Wildlife Disease, biota, rabies, rabies virus, raccoon poxvirus, recombinant, vaccine, Fewer tags Raw MinION FASTQ datafiles corresponding to the paper “A comparison of avian influenza virus whole genome sequencing approaches using nanopore technology” Raw sequencing data as generated by the five different methods used are provided for each of the three samples used in the comparison. The files are in FASTQ format as exported from the Oxford Nanopore’s MK1C using MinION flowcells. Files are labeled according to the method (as described in the paper) and the Sample ID). The MK1C exports data in blocks of 6000 reads per FASTQ file and all the FASTQ files from each method and sample are grouped in a common folder. Categories: Data; Tags: CONUS, Emerging Infectious Disease, Highly Pathogenic Avian Influenza Virus, Methods Comparison, Methods Development, All tags... MinION, Nanopore sequencing, Next Generation Sequencing, Pathogen Discovery, USGS Science Data Catalog (SDC), Wildlife Disease, avian influenza, biota, Fewer tags Microsatellite genotypes and metadata for white-tailed deer samples from the Mid-Atlantic region of USA Dataset containing information for white-tailed deer samples from Ohio, Pennsylvania, Maryland, Virginia and New York, genotyped for 11 microsatellites markers. Marker OvirQ should not be used as it presents alleles inconsistent with reported pattern, with some alleles separated by only 1 base pair and inconsistent between runs. Projected coordinates representing sampling location are in a user-defined CRS, similar to USA Contiguous Albers Equal Area Conic: "+proj=aea +lat_1=29.3 +lat_2=45.3 +lat_0=23 +lon_0=-96 +x_0=0 +y_0=0 +ellps=GRS80 +towgs84=0,0,0,0,0,0,0 +units=m +no_defs". Categories: Data; Tags: Maryland, New York, Ohio, Pennsylvania, USGS Science Data Catalog (SDC), All tags... United States, Virginia, Wildlife Biology, Wildlife Disease, biota, genetics, population and community ecology, wildlife population management, Fewer tags Blacklegged tick nymph densities, tickborne pathogen prevalence, and white-tailed deer densities in eight national parks in the eastern United States from 2014-2022 The data presented here are blacklegged tick nymph densities, white-tailed deer densities, and Borrelia burgdorferi (causative agent of Lyme disease) prevalences for eight national parks in the eastern United States from 2014-2022. Tags: Catoctin Mountain Park, Chesapeake and Ohio Canal National Historical Park, District of Columbia, Ecology, Fire Island National Seashore, All tags... Gettysburg National Military Park, Manassas National Battlefield Park, Maryland, Mid Atlantic, New York, Pennsylvania, Prince William Forest Park, Rock Creek Park, USGS Science Data Catalog (SDC), Virginia, Wildlife Disease, biota, disease vectors, health, wildlife disease, wildlife population management, zoonotic diseases, Fewer tags Data on Utah prairie dog body condition and reproductive success, Awapa Plateau, Utah, USA, 2013-2016 Data on body condition and reproduction of Utah prairie dogs at 5 colonies on the Awapa Plateau, Utah, USA, June-August 2013-2016. Utah prairie dogs were live-trapped and sampled on 5 colonies. We recorded the age (juvenile/adult) and mass (nearest 5 grams) of each prairie dog and marked its ears and body with metal tags and passive integrated transponders, respectively, for permanent identification. We measured each prairie dog's right hind foot length (nearest millimeter). We indexed each adult prairie dog's body condition as the ratio between its mass and hind-foot length. Prairie dogs were allowed to recover from anesthesia and released at their trapping locations. We indexed prairie dog reproduction, by colony... Categories: Data; Tags: Cynomys, Ecology, Prairie dog, USGS Science Data Catalog (SDC), Utah, All tags... Wildlife Biology, Wildlife Disease, biota, capturing (animals), population dynamics, Fewer tags Survey of viral hemorrhagic septicemia virus in Pacific herring throughout the North Pacific Ocean Throughout a 20-year biosurveillance period, viral hemorrhagic septicemia virus was isolated in low titers from only 6 / 7,355 opportunistically sampled adult Pacific herring, reflecting the typical endemic phase of the disease when the virus persists covertly. However, more focused surveillance efforts identified the presence of disease hot spots occurring among juvenile life history stages from certain nearshore habitats. These outbreaks sometimes recurred annually in the same temporal and spatial patterns and were characterized by infection prevalence as high as 96%. Longitudinal sampling indicated that some epizootics were relatively transient, represented by positive samples on a single sampling date, and others... Categories: Data; Tags: Cook Inlet, Ecology, Ketchican, Prince William Sound, Puget Sound, All tags... Sitka Sound, USGS Science Data Catalog (SDC), Wildlife Disease, viruses, Fewer tags Flea abundance and body condition data for black-tailed prairie dogs on sites treated and not treated with “FipBit” fipronil pellets, South Dakota, 2018-2020 Flea abundance and body condition data for black-tailed prairie dogs on sites treated and not treated with “FipBit” fipronil pellets. We sampled prairie dogs at Conata Basin, Buffalo Gap National Grassland, and Badlands National Park, South Dakota, USA. We sampled fleas from live-trapped prairie dogs during June–October 2018, May–October 2019, and July-August 2020. Prairie dogs were sampled before and after FipBit treatments. We anesthetized each prairie dog with isoflurane and combed it thoroughly for 30 seconds to dislodge fleas, which fell into a plastic bin. Fleas were counted and allowed to recover from anesthesia and placed back on prairie dogs, to minimize any removal effect. We weighed prairie dogs with... Categories: Data; Tags: Cynomys, Ecology, Environmental Health, Flea, Plague, All tags... Prairie dog, USGS Science Data Catalog (SDC), Wildlife Biology, Wildlife Disease, Yersinia pestis, biota, disease vectors, Fewer tags Relating tissue egg counts to eggs/g of spleen in Hawaiian green turtles The present study proposes a new methodology for the quantification of parasite eggs in animal tissue. Quantification of parasites are important to understand epidemiology of spirorchiid infections in sea turtles, however different methodologies for quantifying Spirorchiidae eggs in turtle tissues have been used. The most representative way to quantify Spirorchiidae burdens in tissues is counting eggs / g of tissue, however, this method is very laborious. As an alternative, we propose quantifying number of Spirorchiidae egg/ area of tissue on a microscope slide. We compared this method to number of eggs / slide, a common metric of egg burden in turtle tissues. Both methods correlated well with eggs / g with eggs/mm2... Categories: Data; Tags: Hawaii, USGS Science Data Catalog (SDC), Wildlife Disease, climatologyMeteorologyAtmosphere, parasites, All tags... pathology, spirorchiid, Fewer tags Histopathology of tri-colored bats (Perimyotis subflavus) exposed to the fungus Pseudogymnoascus destructans under varying temperature and humidity conditions This work is part of an experimental trial investigating the effects of microclimate conditions of temperature and humidity on a fungal pathogen, Pseudogymnoascus destructans (Pd), that causes white-nose syndrome (WNS) disease in hibernating bats. As part of the trial, tri-colored bats (Perimyotis subflavus) were exposed to Pseudogymnoascus destructans (Pd) and allowed to hibernate in chambers with a variety of temperature and humidity conditions. Bats were euthanized after 83 days. A portion of the wing was rolled around dental wax dowels, fixed in 10% neutral buffered formalin, processed and stained with periodic acid-Schiff, and assessed by light microscopy for evidence of fungal infection. Three types of cutaneous... Categories: Data; Tags: Pseudogymnoascus destructans, Tri-colored bat (Perimyotis subflavus), USGS National Wildlife Health Center, USGS Science Data Catalog (SDC), Wildlife Disease, All tags... atmospheric and climatic processes, biota, hibernation, microclimate, white-nose syndrome, Fewer tags Conventional and quantitative PCR assays for detecting Tetracapsuloides bryosalmonae in fish tissue and environmental DNA water samples We developed and validated conventional and quantitative real-time PCR assays for the detection of DNA from the myxozoan parasite Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in fish. Assays were tested on fish tissue and on field-collected water samples to assess diagnostic and environmental DNA capabilities. The specificity, sensitivity, and broad applicability of the present assays surpass previous methods for detecting T. bryosalmonae DNA from fish tissue and water samples. Types: Citation; Tags: Aquatic Biology, California, Genetics, Idaho, Montana, All tags... Tetracapsuloides bryosalmonae, USGS Science Data Catalog (SDC), Wildlife Disease, Wyoming, environmental DNA, molecular biology, parasites, Fewer tags Data from an investigation of snake fungal disease at Palmetto Island State Park, Vermilion Parish, Louisiana from 2015-2019 This dataset contains the results from 80 visual encounter surveys at two wetlands in Palmetto Island State Park, Vermillion Parish, Louisiana from August 2015 to April 2019. These surveys mainly targeted snakes but small turtles and anoles were also opportunistically captured. Captured animals were swabbed to determine the prevalence of Ophidiomyces ophiodiicola, the causative agent of Snake Fungal Disease. Physical measurements were taken on all captured individuals. This dataset also includes environmental data such as temperature, water level, and humidity at the study site. Categories: Data; Tags: Ecology, Freshwater Ecosystems, Louisiana, North America, Reptiles, All tags... USGS Science Data Catalog (SDC), United States, Vermilion Parish, Wildlife Biology, Wildlife Disease, Wildlife Disease, Fewer tags Biological Indicators and Mercury Concentrations in Smallmouth Bass Biological indicators including morphometric (length, weight), age, sex and health indicators including organismal (health assessment index, condition factor), organ (hepatosomatic index, gonadosomatic index), cellular (intersex, parasite density, macrophage aggregate density), subcellular (plasma vitellogenin, estradiol, testosterone, 11-keto testosterone) and molecular (hepatic transcript abundance) were measured in smallmouth bass from tributaries in the Chesapeake Bay watershed. Total mercury was measured in these fish and correlations between mercury and biological indicators were assessed. Categories: Data; Tags: Antietam Creek, Aquatic Biology, Chillisqaque Creek, Environmental Health, Environmental Health, All tags... Monocacy River, North Branch Potomac River, Pine Creek, South Branch Potomac River, USGS Science Data Catalog (SDC), West Branch Mahantango Creek, Wildlife Disease, Wildlife Disease, biota, endocrine disruptor, fish health, immunity, mercury, reproductive health, Fewer tags Data showing similar movement ecology between mallards infected and not infected with highly pathogenic avian influenza H5N1 These data, which support a paired USGS publication, document the movement and infection status of mallards captured, marked, and tested at a wintering site in Tennessee. Data document differences in movement ecology between mallards infected and not infected with HPAI H5N1. Categories: Data; Tags: Mississippi Flyway, USGS Science Data Catalog (SDC), United States, Wildlife Disease, avian influenza, All tags... biota, movement ecology, Fewer tags Hawaiian forest bird avian malaria prevalence 2018-2021 Native and introduced forest birds were captured and then released across the Hawaiian Islands to acquire a blood sample for obtaining DNA and test for exposure to avian malaria (Plasmodium relictum). A total of 2,945 samples were collected and analyzed for avian malaria prevalence from 39 species captured at 66 sites from Kauai, Oahu, Molokai, Maui, and Hawaii islands. Categories: Data; Tags: Hawai'i Island, Hawaiian forest bird, Kaua'i, Maui, Molokai, All tags... O'ahu, Plasmodium relictum, USGS Science Data Catalog (SDC), Wildlife Biology, Wildlife Disease, avian malaria, biota, disease, Fewer tags Pre- and post-White-nose Syndrome Bat Capture Models These data are the collection of generalized linear mixed models run for AIC comparison of the pre- and post-White-nose Syndrome bat mist-net captures and percent juveniles in capture by year, time since White-nose Syndrome at collection set, U.S Fish and Wildlife Service designated geographic units, states or NABAT grid cell, collection site mean temperature, collection site temperature range and collection site elevation. Models are inclusive of data from 1999-2019 for the little brown bat (Myotis lucifugus), northern long-eared bat (Myotis septentrionalis) and the tri-colored bat (Perimyotis subflavus). Categories: Data; Tags: Ecology, USGS Science Data Catalog (SDC), United States, Wildlife Biology, Wildlife Disease, All tags... bats, biota, Fewer tags Data on flea control using FipBit fipronil bait pellets with black-tailed prairie dogs, South Dakota, 2020-2021 We live-trapped black-tailed prairie dogs on Buffalo Gap National Grassland, South Dakota, 2020-2021. We anesthetized trapped animals (and their fleas) with isoflurane in induction chambers for processing. We combed each individual with a fine-tooth comb for 30 seconds to remove and count fleas. The total flea count from an individual prairie dog was considered an index of flea abundance. We released all animals, after they were recovered from anesthesia, at the location where they were captured. Data were collected on sites treated with fipronil "FipBit" bait pellets for systemic flea control with prairie dogs. Sites were treated with FipBits containing 0.68, 0.71, or 0.83 milligrams of fipronil per FipBit. The... Categories: Data; Tags: Buffalo Gap National Grassland, Cynomys ludovicianus, Ecology, Fipronil, Flea, All tags... Plague, South Dakota, USGS Science Data Catalog (SDC), Wildlife Biology, Wildlife Disease, biota, ecology, Fewer tags Boreal toad survival data in relation to Bd status and community composition These data represent capture mark recapture data from toads, and results of testing for Bd (Batrachochytrium dendrobatidis) the pathogen that causes amphibian chytridiomycosis on individuals. The data span from 2004-2016 at three sites in Montana where boreal toads were declining. The data also include temperature measurements at the sites and information on the egg counts and Bd prevalence from Columbia spotted frogs that are co-occurring at one of the sites. Categories: Data; Tags: Columbia spotted frog, Montana, USGS Science Data Catalog (SDC), Wildlife Disease, biota, All tags... boreal toads, chytridiomycosis, extinction and extirpation, survival, wildlife disease, Fewer tags Data on flea control using fipronil grain bait with black-tailed prairie dogs at Badlands National Park, South Dakota, 2020-2021 We collected fleas from colonies of black-tailed prairie dogs in Badlands National Park, South Dakota, 2020-2021. Specifically, we tested fipronil grain for flea control on two colonies: Colony A in 2020 and Colony B in 2021. To assess on-host flea numbers (in 2020 and 2021), we anesthetized trapped prairie dogs (and their fleas) with isoflurane in induction chambers for processing. We combed each individual with a fine-tooth comb for 30 seconds to remove and count fleas. We released all animals, after they were recovered from anesthesia, at the location where they were captured. To assess off-host flea numbers (in 2021), we used a plumber’s cable to insert a 20- by 20-centimeter flannel-cloth as deep as possible... Categories: Data; Tags: Badlands National Park, Cynomys ludovicianus, Ecology, Fipronil, Flea, All tags... Plague, South Dakota, USGS Science Data Catalog (SDC), Wildlife Biology, Wildlife Disease, biota, ecology, Fewer tags 1 2 3 4 5 ... 8 »