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  4. Chelonid herpesvirus 5 virus isolation data

Chelonid herpesvirus 5 virus isolation data

Dates

Publication Date
Start Date
2008-01-01
End Date
2016-12-31

Citation

Work, T.M., Dagenais, J., Weatherby, T.M., Balazs, G.H., and Ackermann, M., 2018, Chelonid herpesvirus 5 virus isolation data: U.S. Geological Survey data release, https://doi.org/10.5066/F7RJ4GZT.

Summary

Fibropapillomatosis (FP) is a tumor disease of marine turtles associated with Chelonid herpesvirus 5 (ChHV5) that has historically been refractory to growth in tissue culture. Here, we show for the first time de novo formation of ChHV5-positive intranuclear inclusions in cultured green turtle cells, which is indicative for active lytic replication of the virus. The minimal requirements to achieve lytic replication in cultured cells included 1) either in-vitro culturing of ChHV5-positive tumor biopsies or organotypic cultures consisting of ChHV5-positive turtle fibroblasts in collagen rafts seeded with ChHV5-negative or -positive turtle keratinocytes and 2) keratinocyte maturation, which occurred naturally in explanted biopsies or was [...]

Contacts

Point of Contact :
Thierry M Work
Originator :
Thierry M Work, Julie Dagenais, Tina M. Weatherby, George H. Balazs, Mathias Ackermann
Metadata Contact :
Thierry M Work
Distributor :
U.S. Geological Survey - ScienceBase
USGS Mission Area :
Ecosystems
SDC Data Owner :
National Wildlife Health Center

Attached Files

Click on title to download individual files attached to this item.

Chelonid_herpesvirus_5_virus_isolation_data (1).xml
Original FGDC Metadata
View 		37.44 KB application/fgdc+xml
capsid_counts.csv 3.48 KB text/csv
capsid_diameters.csv 23.71 KB text/csv
raft_data.csv 2.77 KB text/csv
time0_eii.csv 291 Bytes text/csv

Purpose

A major challenge in virology is viruses that cannot be grown in the laboratory. One example is chelonid herpesvirus 5 (ChHV5) associated with fibropapillomatosis, a globally distributed, debilitating, and fatal tumor disease of endangered marine turtles. Pathology shows that ChHV5 is shed in skin. Here, we show that ChHV5 will grow in vitro if we replicate the complex three- dimensional structure of turtle skin. Moreover lytic virus growth requires a close interplay between fibroblasts and keratinocytes. Finally, morphogenesis of herpesviral growth in three dimensional cultures reveals a far richer, and likely more realistic, array of capsid morphologies than that encountered in traditional monolayer cell cultures. Our findings have application to other viruses including those of humans.

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  • National Wildlife Health Center
  • USGS Data Release Products

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Type Scheme Key
DOI https://www.sciencebase.gov/vocab/category/item/identifier doi:10.5066/F7RJ4GZT

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