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Chelonid herpesvirus 5 virus isolation data


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Thierry M. Work, Julie Dagenais, Tina M. Weatherby, George H. Balazs, and Mathias Ackermann, 2018, Chelonid herpesvirus 5 virus isolation data: U.S. Geological Survey data release,


Fibropapillomatosis (FP) is a tumor disease of marine turtles associated with Chelonid herpesvirus 5 (ChHV5) that has historically been refractory to growth in tissue culture. Here, we show for the first time de novo formation of ChHV5-positive intranuclear inclusions in cultured green turtle cells, which is indicative for active lytic replication of the virus. The minimal requirements to achieve lytic replication in cultured cells included 1) either in-vitro culturing of ChHV5-positive tumor biopsies or organotypic cultures consisting of ChHV5-positive turtle fibroblasts in collagen rafts seeded with ChHV5-negative or -positive turtle keratinocytes and 2) keratinocyte maturation, which occurred naturally in explanted biopsies or was [...]


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capsid_counts.csv 3.48 KB
capsid_diameters.csv 23.71 KB
Chelonid herpesvirus 5 virus isolation data.xml
Original FGDC Metadata

38.02 KB
plug_data.csv 10.97 KB
raft_data.csv 2.77 KB
time0_eii.csv 291 Bytes


A major challenge in virology is viruses that cannot be grown in the laboratory. One example is chelonid herpesvirus 5 (ChHV5) associated with fibropapillomatosis, a globally distributed, debilitating, and fatal tumor disease of endangered marine turtles. Pathology shows that ChHV5 is shed in skin. Here, we show that ChHV5 will grow in vitro if we replicate the complex three- dimensional structure of turtle skin. Moreover lytic virus growth requires a close interplay between fibroblasts and keratinocytes. Finally, morphogenesis of herpesviral growth in three dimensional cultures reveals a far richer, and likely more realistic, array of capsid morphologies than that encountered in traditional monolayer cell cultures. Our findings have application to other viruses including those of humans.


  • National Wildlife Health Center



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