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Interest in the field of environmental DNA (eDNA) is growing rapidly and eDNA surveys are becoming an important consideration for aquatic resource managers dealing with invasive species. However, in order for eDNA monitoring to mature as a research and management tool, there are several critical knowledge gaps that must be filled. One such gap is the fate of eDNA materials in the aquatic environment. Understanding the environmental factors that influence the decay of eDNA and how these factors impact detection probabilities over time and space could have significant implications for eDNA survey design and data interpretation. Here we experimentally explore eDNA decay in waste materials and reproductive cells obtained...
The vernal pool fairy shrimp, Branchinecta lynchi, (Branchiopoda; Anostraca) and other fairy shrimp species have been listed as ‘threatened’ or ‘endangered’ under the United States’ Endangered Species Act (ESA). There is little information about the sensitivity of Branchinecta spp. to toxic effects of contaminants, making it difficult to determine whether they are adequately protected by water quality criteria. A series of acute (24 hr) lethality/immobilization tests were conducted with three species of fairy shrimp (Branchinecta lynchi, Branchinecta lindahli, and Thamnocephalus platyurus) and 10 chemicals with varying modes of toxic action: ammonia, potassium, chloride, sulfate, chromium(VI), copper, nickel, zinc,...
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Macrhybopsis reproduction and propagule traits were studied in the laboratory using two temperature regimes and three hormone treatments and which methods produced the most spawns. Only sicklefin chub (M. meeki) spawned successfully although sturgeon chub (M. gelida) released unfertilized eggs. All temperature and hormone treatments produced M. meeki spawns, but two treatments had similar success rates at 44 and 43%, consisting of a constant daily temperature with no hormone added, or daily temperature fluctuations with hormone added to the water. Spawns consisted of multiple successful demersal circular swimming spawning embraces interspersed with circular swims without embraces. The most spawns observed for one...
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These replicate data support the findings described in the publication, "A comparison of four pore water sampling methods for mixed metals and dissolved organic carbon, and implications for sediment toxicity evaluations" by Danielle Cleveland, William G. Brumbaugh, and Donald D. MacDonald (Environmental Toxicology and Chemistry 2017). The data represent comparison of four commonly-applied techniques for obtaining pore water samples for the quantification of nickel, copper, zinc, arsenic, cadmium, lead and dissolved organic carbon (DOC), including peepers, push points, centrifugation, and diffusive gradients in thin films (DGTs). The methods were evaluated at low and high concentrations of metals in three sediments...
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Between the years 2000 and 2016, scientists and technicians from the U.S. Geological Survey (USGS) Columbia Environmental Research Center (CERC) have collected over 400 field-days worth of acoustic Doppler current profiler (ADCP) measurements on the Missouri and Yellowstone Rivers, primarily for the purposes of assessing physical aquatic habitat for the pallid sturgeon. Scientists and technicians collected data using boat-mounted Teledyne Rio Grande ADCPs, which were processed using customized scripting tools and archived in standardized formats. To assess longitudinal variability in depth and velocity distributions along the Missouri River, as well as compare the Missouri River to its unaltered analog, the Yellowstone...
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This data was generated from a study in which five experiments were conducted that tested whether and how dissolved chemicals might assist food recognition in two filter-feeding fishes, the silver (Hypophthalmichthys molitrix) and the bighead carp (H. nobilis). The buccal-pharngeal pumping (BPP), a behavior in which fish pump water into their buccal cavities, was observed in both silver and bighead carps after exposure to a a variety of food filtrates and mixtures. In addition, occlusion experiments to determine if the olfactory sense has a very important, but not exclusive, role in bigheaded carp feeding behaviors were conducted.
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We measured ontogenetic changes in routine and maximum swimming speeds of bighead, grass, and silver carp larvae. Daily measurements of routine swimming speed were taken for two weeks post-hatch using a still camera and the LARVEL program, a custom image-analysis software. Larval swimming speed was calculated using larval locations in subsequent image frames and time between images. Using an endurance chamber, we determined the maximum swimming speed of larvae (post gas bladder inflation) for four to eight weeks post-hatch.
The risks to wildlife and humans from uranium (U) mining to the Grand Canyon watershed are largely unknown. In addition to U, other co-occurring ore constituents contribute to risks to biological receptors depending on their toxicological profiles. This data was collected to characterize the pre-mining concentrations of total arsenic (As), cadmium (Cd), copper (Cu), lead (Pb), mercury (Hg), nickel (Ni), selenium (Se), thallium (Tl), U, and zinc (Zn); radiation levels; and histopathologies in biota (vegetation, invertebrates, amphibians, birds, and mammals) at the Canyon Mine.
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This data release contains chemistry and toxicity data from sediment toxicity tests conducted by the USGS Columbia Environmental Research Center (CERC) with 66 sediment samples collected from in and around the Upper Columbia River in the fall of 2013. Toxicity testing was conducted from fall 2013 through summer 2014 with the amphipod, Hyalella azteca, the midge Chironomus dilutus, and the mussel Lampsilis siliquoidea. Short-term toxicity endpoints (10-28 d) included survival, weight, and biomass of all test organisms. Long-term tests with amphipods (42 d) and midges (about 50 d) included reproduction endpoint. Sediments were analyzed for physical and chemical characteristics, including particle size distribution,...
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This dataset includes microsomal EROD data from an assay done with liver samples from several fish species that are found in Arizona at sites that are being assessed for PBDE contamination. The data was created in September and October 2016.
The data consists of a summary of amphibian acoustic recordings at Canyon, Arizona 1, and Pinenut mines near the Grand Canyon. USGS is currently conducting biological surveys associated with uranium mines on federal lands in Arizona. These surveys include determining the composition of the local amphibian community. Original raw acoustic recordings used to create this summary data table are archived at Columbia Environmental Research Center.
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Advances in drilling technique have facilitated a rapid increase in hydrocarbon extraction from energy shales, including the Williston Basin in central North America. This area overlaps with the Prairie Pothole Region and greater than 35% of wetlands are less than or equal to 1 km from a petroleum-related well. Legacy practices often released saline co-produced waters (brines) that were chloride rich wastes, affecting wetland water quality directly or persisting in sediments. Despite the potential threat of brine contamination to aquatic habitats, there has been little research into their ecological effects. We capitalized on a gradient of legacy brine-contaminated wetlands in northeast Montana to conduct laboratory...
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Invasive crayfish are known to displace native crayfish species, alter aquatic habitat and community structure and function, and are serious pests for fish hatcheries. White River Crawfish (WRC; Procambarus acutus) were inadvertently introduced to a warm-water fish hatchery in Missouri, USA, possibly in an incoming fish shipment. We evaluated the use of chemical control for crayfish to ensure incoming and outgoing fish shipments from hatcheries do not contain live crayfish. We conducted acute (less than or equal to 24 hr) static toxicity tests to determine potency, dose-response, and selectivity of pesticides to WRC, Virile Crayfish (VC; Orconectes virilis), and Fathead Minnow (FHM; Pimephales promelas). Data included...
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Following an explosion at the Deepwater Horizon drilling rig on April 20, 2010, MC252 an estimated 3.19 million barrels of oil leaked into the Northern Gulf of Mexico over 87 days. The endangered Kemp's ridley sea turtle (Lepidochelys kempii [Lk]) has a long established record of using the area near the well as primary foraging grounds. Resulting short- and long-term negative impacts of the oil spill on sea turtles could induce changes in the health and fitness of the Lk population. MC252 oil is known to have entered the foraging areas. Consequently, stable carbon and nitrogen isotope signatures from MC252 oil should be reflected in the food web and in the tissues of organisms such as sea turtles. We measured carbon...
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Invasive Asian carps established in the United States spawn in turbulent water of rivers and their eggs and early larvae develop while drifting in the current. The eggs are slightly denser than water and are held in suspension by water turbulence. The eggs are believed to perish if they settle before hatching. It is thus possible to use egg drift modeling to assess the capability of a river to support survival of Asian carp eggs. Data to populate such models include the physical properties of the assessed rivers, and information on egg size, density, and terminal fall velocity (sinking rates). Herein, we present the physical characteristics of the eggs as a function of post fertilization time. We recorded mean egg...
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Invasive silver carp (Hypophthalmichthys molitrix) occur in large regions of the Mississippi River drainage, outcompete native species, and are notorious for their prolific jumping behavior. Juvenile and adult (up to 25 kg) carp are known to jump up to 3 m above the water surface in response to moving watercraft. Broadband sound recorded from an outboard motor (100 hp at 32 km/hr) can modulate their behavior in captivity; however, the response of wild silver carp to broadband sound has yet to be determined. This data set includes information regarding the sound produced by outboard motor and speakers in the field and will help in determining effectiveness of the usage of control techniques in the management of carps.
Data set contains depth and velocity grids centerpoints generated from hydrodynamic model (SRH-2D) simulations run for a range of discharges. Date range corresponds to month(s) that bathymetric survey was performed. Three study reaches were considered in this analysis: the Fairview reach of the Yellowstone River, MT, the Miami and Lisbon-Jameson reaches of the Lower Missouri River. Data and application are described in detail in accompanying journal article (Erwin et al., 2017).


    map background search result map search result map Data in support of manuscript "Evaluation of chemical control for invasive crayfish at a warmwater fish production hatchery" Data tables in support of manuscript "​δ13C and δ15N in the endangered Kemp's ridley sea turtle lepidochelys kempii after the Deepwater Horizon oil spill" Acoustic Doppler current profiler raw measurements on the Missouri and Yellowstone rivers, 2000-2016, Columbia Environmental Research Center Data for "Comparison of size, terminal fall velocity and density of bighead, silver, and grass carp eggs for use in drift modeling" Microsomal EROD data of fish liver sample assay from species collected in the Salt and Gila Rivers, Arizona Reproductive strategy, spawning induction, spawning temperatures and early life history of captive sicklefin chub Macrhybopsis meeki-Data Physical, chemical and bioassay data from an evaluation of metal-contaminated sediments and pore water from the Upper Columbia River, Washington, 2013-2014 Widespread legacy brine contamination from oil shales reduces survival of chorus frog larvae-Data A comparison of four pore water sampling methods for mixed metals and dissolved organic carbon, and implications for sediment toxicity evaluations-Data Ontogenetic changes in swimming speed of silver carp, bighead carp, and grass carp larvae-Data Chemical cues which include amino acids mediate species-specific feeding behavior in invasive filter-feeding bigheaded carps-Data Asian carp acoustic stimuli data Widespread legacy brine contamination from oil shales reduces survival of chorus frog larvae-Data Physical, chemical and bioassay data from an evaluation of metal-contaminated sediments and pore water from the Upper Columbia River, Washington, 2013-2014 Data tables in support of manuscript "​δ13C and δ15N in the endangered Kemp's ridley sea turtle lepidochelys kempii after the Deepwater Horizon oil spill" Acoustic Doppler current profiler raw measurements on the Missouri and Yellowstone rivers, 2000-2016, Columbia Environmental Research Center