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This data set includes geospatial data and tables providing location, environmental, and vegetation data collected in 2017 and 2018 at the Little Saint Francis River (LSFR) chat pile restoration site, Fredericktown, Madison County, Missouri. Restoration actions are being implemeneted as part of the settlement for the Madison County Mines Superfund site to compensate the public for losses of natural resources and the services they provide as part of the Natural Resource Damage Assessment and Restoration Southeast Missouri Lead Mining District case. Data were collected prior to and during the early stages of restoration actions to restore bottomland forest habitat, reduce invasive plant species abundance, and improve...
Within large-river ecosystems, floodplains serve a variety of important ecological functions. A recent survey of 80 managers of floodplain conservation lands along the Upper and Middle Mississippi and Lower Missouri Rivers in the central United States found that the most critical information needed to improve floodplain management centered on metrics for characterizing depth, extent, frequency, duration, and timing of inundation. These metrics can be delivered to managers efficiently through cloud-based interactive maps. To calculate these metrics, we interpolated an existing one-dimensional HEC-RAS hydraulic model for the Middle Mississippi River, which simulated water surface elevations at cross sections spaced...
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Macrhybopsis reproduction and propagule traits were studied in the laboratory using two temperature regimes and three hormone treatments and which methods produced the most spawns. Only sicklefin chub (M. meeki) spawned successfully although sturgeon chub (M. gelida) released unfertilized eggs. All temperature and hormone treatments produced M. meeki spawns, but two treatments had similar success rates at 44 and 43%, consisting of a constant daily temperature with no hormone added, or daily temperature fluctuations with hormone added to the water. Spawns consisted of multiple successful demersal circular swimming spawning embraces interspersed with circular swims without embraces. The most spawns observed for one...
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The Columbia Environmental Research Center (CERC) is a U.S. Geological Survey (USGS) environmental science research facility located in Columbia, Missouri, USA. CERC conducts national and international environmental contaminant research and investigates effects of habitat alterations on aquatic and terrestrial ecosystems.
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This data was generated from a study in which five experiments were conducted that tested whether and how dissolved chemicals might assist food recognition in two filter-feeding fishes, the silver (Hypophthalmichthys molitrix) and the bighead carp (H. nobilis). The buccal-pharngeal pumping (BPP), a behavior in which fish pump water into their buccal cavities, was observed in both silver and bighead carps after exposure to a a variety of food filtrates and mixtures. In addition, occlusion experiments to determine if the olfactory sense has a very important, but not exclusive, role in bigheaded carp feeding behaviors were conducted.
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This child data release includes hyperspectral and RGB images acquired from an Unmanned Aircraft System (UAS) during an experiment performed at the USGS Columbia Environmental Research Center, near Columbia, Missouri, on April 2, 2019. The purpose of the experiment was to assess the feasibility of inferring concentrations of a visible dye (Rhodamine WT) tracer from various types of remotely sensed data in water with varying levels of turbidity. Whereas previous research on remote sensing of tracer dye concentrations has focused on clear-flowing streams, the Missouri River is much more turbid and the reflectance signal associated with the sediment-laden water could obscure that related to the presence and amount...
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The data provide location and data quality information for ground control points (GCP) deployed at Palmyra Atoll for acquisition of imagery using small unoccupied aerial systems (sUAS) in October 2016. Thales ProMark 3 handheld geographic positioning systems (GPS) were used as both a local base station and to record locations of individual GCPs, with occupancy times of approximately 30 minutes per GCP. Location data for GCPs were post-processed against base station data using Mobile Mapper Office software to yield local position accuracy of approximately 0.1 m.
Within large-river ecosystems, floodplains serve a variety of important ecological functions. A recent survey of 80 managers of floodplain conservation lands along the Upper and Middle Mississippi and Lower Missouri Rivers in the central United States found that the most critical information needed to improve floodplain management centered on metrics for characterizing depth, extent, frequency, duration, and timing of inundation. These metrics can be delivered to managers efficiently through cloud-based interactive maps. To calculate these metrics, we interpolated an existing one-dimensional HEC-RAS hydraulic model for the Middle Mississippi River, which simulated water surface elevations at cross sections spaced...
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Point clouds, high resolution digital surface models (DSM), and high resolution orthoimagery of Palmyra Atoll, USA, were generated from aerial 3-color and 5-band imagery collected October 2016 using Unoccupied Aerial Systems (UAS, or "drones"). Data were processed in sections corresponsing to flight areas using photogrammetry to generate three-dimensional point clouds (.las) by identifying pixels from multiple images representing the same object and calculating the x, y, and z coordinates of that object/pixel. Point clouds for each section were processed to create DSMs (.tif) representing the continuous surface of the uppermost reflective surface (i.e., data have not been filtered for vegetation classes or height)....
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We measured ontogenetic changes in routine and maximum swimming speeds of bighead, grass, and silver carp larvae. Daily measurements of routine swimming speed were taken for two weeks post-hatch using a still camera and the LARVEL program, a custom image-analysis software. Larval swimming speed was calculated using larval locations in subsequent image frames and time between images. Using an endurance chamber, we determined the maximum swimming speed of larvae (post gas bladder inflation) for four to eight weeks post-hatch.
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This dataset includes microsomal EROD data from an assay done with liver samples from several fish species that are found in Arizona at sites that are being assessed for PBDE contamination. The data was created in September and October 2016.
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This child data release includes in situ measurements of turbidity and Rhodamine WT dye concentration acquired during an experiment performed at the USGS Columbia Environmental Research Center, near Columbia, Missouri, on April 2, 2019. The purpose of the experiment was to assess the feasibility of inferring concentrations of a visible dye (Rhodamine WT) tracer from various types of remotely sensed data in water with varying levels of turbidity. Whereas previous research on remote sensing of tracer dye concentrations has focused on clear-flowing streams, the Missouri River is much more turbid and the reflectance signal associated with the sediment-laden water could obscure that related to the presence and amount...
Within large-river ecosystems, floodplains serve a variety of important ecological functions. A recent survey of 80 managers of floodplain conservation lands along the Upper and Middle Mississippi and Lower Missouri Rivers in the central United States found that the most critical information needed to improve floodplain management centered on metrics for characterizing depth, extent, frequency, duration, and timing of inundation. These metrics can be delivered to managers efficiently through cloud-based interactive maps. To calculate these metrics, we interpolated an existing one-dimensional HEC-RAS hydraulic model for the Middle Mississippi River, which simulated water surface elevations at cross sections spaced...
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Grass carp, bighead carp, and silver carp spawn in flowing water. Their eggs,and then larvae, develop while drifting. Hydraulic conditions control spawning locations, egg survival, and the downstream distance traveled before the hatched larvae can swim for low velocity nursery habitats. Existing egg drift models simulate the fluvial transport of carp eggs but have limitations in capturing the effect of localized turbulence on egg transport due to inadequate dimensions of hydrodynamics and/or empirical parameterization of river dispersion. We present a three-dimensional Lagrangian particle tracking model that uses fully resolved river hydrodynamics and a continuous random walk algorithm driven by local turbulent...
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Description of Work Determine the relative sensitivity of two species of amphibians (i.e. Wood Frogs Rana sylvatica, Cricket Frogs Acris crepitans blanchardi, American Toad Bufo americana) to exposure to acute and chronic levels of nitrate, nitrite and ammonia. This data will be used to evaluate the current ammonia criteria and for possible inclusion in the development of criteria for nitrite and nitrate.
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We have adapted two commonly used human breast luciferase transactivation cell bioassays, the recently re-named estrogen agonist/antagonist screening VM7Luc4E2 cell bioassay (previously designated BG1Luc4E2) and the androgen/glucocorticoid screening MDA-kb2 cell bioassay, to 384-well formats for HTS of endocrine-active substances. This cost-saving adaptation includes a fast, accurate, and easy measurement of protein amount in each well via the fluorescamine assay (in the form of relative fluorescence units, RFU which are then converted to microgram protein using bovine serum albumin standards) with which to normalize luciferase activity (in the form of relative light units, RLU) of cell lysates without requiring...
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This dataset contains cross-sectionally averaged flow metrics from measurements made with acoustic Doppler current profilers (ADCP) on the Missouri River near Wolf Point, MT during 2018 and 2019. Each data point represents a single-pass measurement with a boat-mounted ADCP driven from bank-to-bank along lateral transects spaced ~15 meters apart along a 33 kilometer reach. The measurements were collected as part of a bathymetric survey in which two USGS survey boats were each mounted with an ADCP and single beam echosounder that were deployed simultaneously. Cross-sectionally averaged flow metrics such as velocity, depth, and temperature along with the total measured discharge and starting coordinate of the measurement...
This dataset consists of seven geomorphic variables attributed to standard river mile address points at 0.1 mile (160 m) intervals between Fort Peck Dam and the headwaters of Lake Sakakawea. The data illustrate the geomorphic variation in this 300-km segment of the Upper Missouri River and are indicative of channel complexity, thought to be an important influence on dispersal and retention of larval sturgeon.
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Description of Work The work completed for this template will provide the following information: 1) Generate acute and chronic toxicity data for freshwater mussels and snails to enhance the protection of listed/endangered species in the GL basin; 2) Refine methods and conduct acute and chronic toxicity tests on amphibians; 3) Develop and test methods for conducting chronic toxicity tests for mayflies.


map background search result map search result map Columbia Environmental Research Center (CERC) Determination of the Sensitivity of Two Species of Amphibians to Toxicity From Nitrate, Nitrite and Ammonia Toxicity Testing for Stream Dwelling Amphibians, Mussels, and Mayflies Adaptation and establishment of microchemical methods to analyze elemental patterns in calcified structures of sturgeon for determining origins and movements of freshwater sturgeon in the Mississippi and Missouri river basins Development of a dual luciferase-fluorescamine assay adapted to a 384 micro-well plate format-Data Microsomal EROD data of fish liver sample assay from species collected in the Salt and Gila Rivers, Arizona Reproductive strategy, spawning induction, spawning temperatures and early life history of captive sicklefin chub Macrhybopsis meeki-Data Ontogenetic changes in swimming speed of silver carp, bighead carp, and grass carp larvae-Data Chemical cues which include amino acids mediate species-specific feeding behavior in invasive filter-feeding bigheaded carps-Data Pre-restoration vegetation data, Little Saint Francis River chat pile site, Missouri, USA, 2017 and 2018 Orthoimagery and elevation data derived from UAS imagery for Palmyra Atoll, USA 2016 (ver. 1.1, November 2020) Hyperspectral and RGB images acquired during an experiment conducted at the USGS Columbia Environmental Research Center, Columbia, MO, on April 2, 2019 In situ measurements of turbidity and Rhodamine WT dye concentration from an experiment conducted at the USGS Columbia Environmental Research Center, Columbia, MO, on April 2, 2019 Orthoimagery and elevation data derived from UAS imagery for Palmyra Atoll, USA 2016-GCPs 2016 No Levee (Altered) Inundation Metrics for the Middle Mississippi River With Levee (baseline) Inundation Metrics for the Middle Mississippi River Base Terrain and Bathymetry for the Middle Mississippi River Cross-sectionally averaged flow metrics from ADCP measurements of the Missouri River downstream of Wolf Point, MT during 2018-2019 Data supporting a new three-dimensional Lagrangian particle tracking model for predicting transport of carp eggs in turbulent rivers Hyperspectral and RGB images acquired during an experiment conducted at the USGS Columbia Environmental Research Center, Columbia, MO, on April 2, 2019 In situ measurements of turbidity and Rhodamine WT dye concentration from an experiment conducted at the USGS Columbia Environmental Research Center, Columbia, MO, on April 2, 2019 Pre-restoration vegetation data, Little Saint Francis River chat pile site, Missouri, USA, 2017 and 2018 Orthoimagery and elevation data derived from UAS imagery for Palmyra Atoll, USA 2016-GCPs 2016 Data supporting a new three-dimensional Lagrangian particle tracking model for predicting transport of carp eggs in turbulent rivers Orthoimagery and elevation data derived from UAS imagery for Palmyra Atoll, USA 2016 (ver. 1.1, November 2020) Base Terrain and Bathymetry for the Middle Mississippi River No Levee (Altered) Inundation Metrics for the Middle Mississippi River With Levee (baseline) Inundation Metrics for the Middle Mississippi River Adaptation and establishment of microchemical methods to analyze elemental patterns in calcified structures of sturgeon for determining origins and movements of freshwater sturgeon in the Mississippi and Missouri river basins Toxicity Testing for Stream Dwelling Amphibians, Mussels, and Mayflies Determination of the Sensitivity of Two Species of Amphibians to Toxicity From Nitrate, Nitrite and Ammonia